ALMS1-Deficient Fibroblasts Over-Express Extra-Cellular Matrix Components, Display Cell Cycle Delay and Are Resistant to Apoptosis
Figure 4
ALMS fibroblasts display a longer cell cycle than controls.
Cell cycle length of control (C1–C3) and ALMS fibroblasts (PT1–PT4) was estimated by culture experiments in 10% FBS SM (white symbols), in 2% FBS SM (black symbols), and upon serum deprivation for 48 hour, followed by culture in 10% FBS SM (grey symbols) by comparing the number of viable cells at 0 hours and at 72 hours of culture, using CellTiter-Glo® Luminescent Cell Viability Assay (Promega). The short dash line indicates the mean value of cell cycle length of controls in all growth conditions analyzed, while the dotted lines represent the mean value ± one SD.