Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
Figure 6
Mice lacking T cells are as susceptible to primary VACV infection as Rag1−/− mice.
NUDE athymic mice (nu/nu) and their wild type littermates (nu/+) were infected with 107 p.f.u. of VACV-WR by tail scarification. (A) Weight curve of NUDE mice (***p<0.001, unpaired t test). (B) Survival curve of nu/nu and nu/+ after infection (***p<0.001, log-rank test). (C) Viral titers were determined in tail of C57BL/6 and nu/nu mice (**p<0.01 day 10 p.i. and *p<0.05, t test). Data of viral titer in tails of nu/+ mice are missing. (D) Spleens of nu/nu and nu/+ mice were removed at the times indicated and virus titer was determined in BSC-40 cells. (E) VACV-specific IgG antibodies were determined by ELISA in serum of nu/nu and nu/+ mice (**p<0.01 and ***p<0.001, t test). Bars represent the mean and standard error (SEM), except for 6E, where bars represent mean and standard deviation (SD). The results shown are representative of two experiments. ND: not detected.