RNA Captor: A Tool for RNA Characterization
Figure 5
Comparison of Rnl2, Dnl and Rnl1.
Oligonucleotide tagging assays were carried out with the ligase, substrate and temperature shown on each lane, in 30% (Dnl and Rnl2) or 25% (Rnl1) PEG ligation buffer. The substrates are described in figure 2. Rnl2 outperforms Dnl and Rnl1 for adding an adapter on the 5′ end of RNAs.