Cooperative Nuclear Localization Sequences Lend a Novel Role to the N-Terminal Region of MSH6
Figure 5
The full kinetic model for import of MSH6 into the nucleus with 12 species and 14 reactions, is shown diagrammatically.
Each green ball is a molecular species or complex and each yellow oval is a reaction. The arrows into a yellow oval indicate those species are reactants and errors out indicate the species are products. The different species and reaction types are organized spatially for clarity. Each of the four base species, MSH6, and the binders to NLS1, NLS2, and NLS3 (bNLS1, bNLS2, bNLS3, respectively, are shown at the bottom as green circles as species that are reactants for the formation reactions to which they are linked by inward arrows. The formation reactions that lead to complex formation are shown in yellow in the middle, with the complexes formed shown at top as green circles; the complexes formed are linked to the correct formation reactions by outward arrows. The complexes are named appropriately, so that for example, MSH6_b1 is the complex of MSH6 with the binder to NLS1 and is connected via a yellow reaction oval to bNLS1 (the binder to NLS1) and MSH6 in the cytoplasm. The disintegration reactions that implicitly model transport into the nucleus through complex disintegration are shown at top right in yellow, where they connect the appropriate complex to the correct molecular species, leading to MSH6 in the nucleus. For example, MSH6_b1 is connected to MSH6 in the nucleus and bNLS1 (the binder to NLS1) via a disintegration reaction.