The Role of Neutrophils during Mild and Severe Influenza Virus Infections of Mice
Figure 5
Virus replication in neutropenic and control mice following infection with BJx109, HKx31 and PR8.
Groups of 5 B6 mice received i.n. and i.p. treatments with purified anti-Ly6G (1A8, striped bars) 24 hrs prior to virus infection and every second day thereafter. For comparison, virus-infected mice were also treated with anti-Gr-1 (RB6-8C5, grey bars) antibodies and control mice with rat IgG (IgG, black bars). Mice were infected with 105 PFU of BJx109, HKx31 or PR8 and at day 5 post-infection mice were killed, lungs removed and titres of infectious virus were determined in clarified homogenates by standard plaque assay. Data represent mean viral titres titres ± 1 SD. The detection limit for the assay is indicated by the dotted line. Viral titres from treated mice (RB6-8C5 or 1A8) that were significantly higher than those from control (IgG-treated) animals are indicated by * = p<0.05, ** = p<0.01 (Student's t-test).