Integrated Analysis of Gene Expression, CpG Island Methylation, and Gene Copy Number in Breast Cancer Cells by Deep Sequencing
Figure 2
The mRNA-seq data validate in comparison to qPCR and NanoString data.
Panel A: A comparison of the abundance of three transcripts (ESR1, PGR, and ERBB2) measured by mRNA-seq (blue symbols) or qPCR (red symbols). Panel B: A correlation plot between mRNA-seq and NanoString for 236 cancer reference genes. Log2 RPM data for mRNA-seq and log2 NanoString data were used. R was used to calculate the Pearson correlation coefficient. Panel C: log2 fold change correlation between mRNA-seq and NanoString for 25 differentially expressed genes detected by NanoString.