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Drop-on-Demand Single Cell Isolation and Total RNA Analysis

Figure 2

Patterned droplets.

(a) Schematic droplet subarray showing four different cases of patterned droplets. Green colored circles indicated target cells. (b) Portion of a droplet array showing examples of each of the four different cases of patterned droplets, 1) single target cell droplet, 2) an empty droplet without cells, 3) a non-target cell droplet, and 4) a heterogeneous droplet containing target and non-target cells. Green fluorescent stained cells were target cells. Scale bars, 200 µm.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0017455.g002