A Survey of New Temperature-Sensitive, Embryonic-Lethal Mutations in C. elegans: 24 Alleles of Thirteen Genes
Figure 5
A. DIC time-lapse images of wild-type, mei-1(or642 ts) and mei-1(or646 ts) embryos. In the mei-1 mutants the polar bodies were large and misshapen and embryos contained multiple [top mei-1(or642 ts) embryo and mei-1(or646 ts)] or zero maternal pronuclei (second mei-1(or642 ts) embryo). The two mei-1(or642 ts) embryos were obtained from a hermaphrodite shifted to the restrictive temperature for 30 minutes, the mei-1(or646 ts) embryo was obtained from a hermaphrodite shifted to the restrictive temperature for 7 hours prior to imaging. White arrowheads indicates polar bodies, black arrowheads indicate multiple maternal pronuclei, the black arrow denotes multiple nuclei per cell at the two cell stage, and the “p” refers to the paternal pronucleus in an embryo lacking a maternal pronucleus. Times in min:sec are given relative to nuclear envelope breakdown (NEBD). Scale bar, 10 µm. B. Defect maps of individual embryos observed during time-lapse recordings: embryos are listed on the left and phenotypes are listed on the top: 1; normal polar body size, 2; normal pronuclear number, 3; one nucleus per cell at two cell stage. In the long upshifts, hermaphrodites were transferred to the restrictive temperature for 5–8 hours. In the short upshifts, embryos were harvested from hermaphrodites grown at the restrictive temperature for 30 minutes. C. Amino acid alteration in the mutants. Asterisk indicates the changed residue. Homologous proteins are aligned below the C. elegans protein.