Isolation of a Rickettsial Pathogen from a Non-Hematophagous Arthropod
Figure 3
Propagation of Rickettsia isolated from booklice in ISE6 cells.
(A) ISE6 cells infected with Rickettsia isolated from booklice were Cytospin centrifuged and rickettsiae were visualized by Diff-Quik staining. (B) The infected cells on coverslips were fixed and permeabilized prior to stain with mouse polyclonal antibody against R. felis and FITC-conjugated goat anti-mouse IgG. Host cell actin and nuclei were stained with with Rhodamine-Phalloidin and DAPI as shown in red and blue, respectively. Bar = 5 µm.