Isolation of a Rickettsial Pathogen from a Non-Hematophagous Arthropod
Figure 2
Electron micrographs of Rickettsia in booklice.
(A) Rickettsiae free in the cytosol of gut epithelial cells. MV represents microvilli. (B) Higher magnification of Fig. 2A shows typical rickettsial ultrastructure: rickettsial cell wall including trilaminar cell wall membrane associated with the external surface microcapsule layer and an internal trilaminar cytoplasmic membrane (arrowheads), surrounded by an outermost “halo” zone (h). Solid arrow indicates a small vacuole inside rickettsial cytoplasm. (C) Rickettsiae (R) in ovary. Inset: higher magnification view of the boxed rickettsiae. (D) Ultrathin section of a mycetome-like, several cells of which contain large vacuoles tightly packed with rickettsiae of irregular shape with dense cytoplasm. Bar = 500 nm.