Aureusimines in Staphylococcus aureus Are Not Involved in Virulence
Figure 7
Sae mutants can be enriched under typical staphylococcal mutagenesis conditions.
Transposon insertion mutants of geh (glycerol ester hydrolase) and saeR were used in this test. Equal numbers of the two mutants were mixed and grown either in the absence or in the presence of erythromycin (10 µg/ml) with shaking for 16 h at 43°C. Chromosomal DNA was purified from each culture and the fold change of each strain was calculated by quantitative real time PCR (qPCR) as described in Materials and Methods. Bar graphs indicate the fold change of each strain. Data are presented as the mean ± standard deviation from three independent experiments. Input, the mixed culture used for inoculation; - Erm, culture grown in the absence of erythromycin; + Erm, culture grown in the presence of erythromycin.