Aureusimines in Staphylococcus aureus Are Not Involved in Virulence
Figure 5
The sae deletion mutant is more resistant to certain antibiotics.
A. Effect of the sae deletion mutation on resistance to chloramphenicol and erythromycin at 37°C or 43°C. Cells were inoculated in 200 µl TSB; then the growth of the cells was measured with Bioscreen C (Growth Curves USA) for 24 h. The antibiotics and growth temperatures are shown above each graph. The concentration (µg/ml) of antibiotics is shown in parentheses. B. Effect of the sae deletion mutation on resistance to ciprofloxacin and oxacillin at 37°C. C. Complementation test for the sae deletion mutant. The used erythromycin concentration is indicated to the right of the graph. pCL55, an integration vector; pCL55-sae, pCL55 containing the entire sae operon.