Aureusimines in Staphylococcus aureus Are Not Involved in Virulence
Figure 3
The SaeRS TCS is functional in the ausA transposon mutant ΦΝΞ-12202.
A. Hemolysis patterns on sheep blood agar. The strains tested were streaked against the strain RN4220, which produces only beta-hemolysin. The white arrow indicates the hemolysis caused by alpha-hemolysin. The enhanced hemolysis at the junctions of strain RN4220 and the tested strains is due to delta-hemolysin production originating from the test strains. NM, wild type; 12202, transposon insertion mutant ΦΝΞ-12202; Δsae, a deletion mutant of the sae operon; ΔausA, original ausA deletion mutant; pCL55-sae, pCL55 plasmid containing the entire sae operon. B. Western blot analysis for alpha-hemolysis (Hla) and SaeP protein. NM, wild type; 12202, ΦΝΞ-12202; ΔsaeP, a transposon insertion mutant of saeP; Δhla, a transposon insertion mutant of hla, the gene encoding alpha-hemolysin. C. LacZ assay for P1 promoter activity. Mid-log phase cells were used to measure LacZ activity from P1-lacZ fusion in strains Newman (NM), ΦΝΞ-12202, and the sae deletion mutant (Δsae). Bar graphs depict the mean ± standard deviation for the relative LacZ activity of each indicated strain.