Aureusimines in Staphylococcus aureus Are Not Involved in Virulence
Figure 2
The ausA transposon mutant ΦΝΞ-12202 does not produce aureusimines.
A. The ausAB operon in the transposon mutant ΦΝΞ-12202. The transposon insertion is indicated by a reversed triangle. The primers (PM1, PM2, and PM3), used for PCR-amplification, are shown as arrows. B. PCR amplification of the ausA region of wild-type Newman (W) and the transposon insertion mutant ΦΝΞ-12202 (M). The numbers at the top represent the primer pairs. The calculated size of each product is indicated on the right of the picture. Molecular size markers are shown to the left. C. The absence of aureusimines in the culture supernatant of the transposon mutant ΦΝΞ-12202. The culture supernatants from wild-type Newman and the transposon mutant were extracted with ethyl acetate and suspended in methanol. The supernatant extracts were analyzed with HPLC. As a control, synthetic aureusimine A was used. A, aureusimine A; B, aureusimine B.