Heterogeneous Response to a Quorum-Sensing Signal in the Luminescence of Individual Vibrio fischeri
Figure 2
Individual V.fischeri imaged in dark field and bioluminescence.
(A) Dark field (externally illuminated) and (B) bioluminescence (light emission) images of V.fischeri cells adhered to the glass window of the perfusion chamber at 24°C in the presence of 500 nM exogenous AI. The cells appear as rods (∼3–5 µm long) in the dark field image. The bioluminescence image shows in false color the luminescent emission detected in a 16 minute exposure. Images were collected in an inverted microscopy configuration with an intensified CCD camera and a 100× oil immersion objective.