Dynamic vs Static ABCG2 Inhibitors to Sensitize Drug Resistant Cancer Cells
Figure 6
Effect of PZ-34 and PZ-38 on ABCG2 stability.
A, Western blot analysis. HEK293/ABCG2 cells were first treated with 5 µg/ml cycloheximide (CHX) followed by addition of 3 µM of PZ-34, PZ-38, FTC, or DMSO control for various times. The cells were then harvested for Western blot analysis of ABCG2 probed with monoclonal antibody BXP-21. Actin was used as a loading control. B, half-life of ABCG2. ABCG2 levels on Western blot as shown in panel A were determined using Scion Image and plotted against time of treatment. Data shown are mean± S.D of three experiments.