In Vitro and In Vivo Anti-Inflammatory Activity of 17-O-Acetylacuminolide through the Inhibition of Cytokines, NF-κB Translocation and IKKβ Activity
Figure 13
Effect of 17-O-acetylacuminolide (AA) on serum TNF levels in mice.
Mice were pretreated i.p. with either 100 mgkg−1 of AA, 6 mgkg−1 of dexamethasone (DEX) or with Phosphate buffer saline (PBS) and DMSO (untreated) for 30 mins. The mice were then either injected with 1 mgkg−1 lipopolysaccharide (LPS) or with PBS for 90 mins. Blood was withdrawn, and serum TNF was quantified using ELISA. One way ANOVA with Tukey's post analysis was used to calculate the statistical significance among the groups when compared to PBS+LPS group; *** p<0.001.