In Vitro and In Vivo Anti-Inflammatory Activity of 17-O-Acetylacuminolide through the Inhibition of Cytokines, NF-κB Translocation and IKKβ Activity
Figure 10
Effect of AA on LPS-stimulated RAW264.7 cells.
NF-κB (DyLight™ 488, light green), was sequestered in the cytoplasm in cells treated with DMEM alone (untreated), and the nuclei of the cells appear blue (Hoescht). However, NF-κB translocates into the nucleus upon LPS stimulation. Pretreatment with 17-O-acetylacuminolide (AA) was able to prevent NF-κB translocation in the presence of LPS (IC50 = 7.8 µgmL−1).