In Vitro and In Vivo Anti-Inflammatory Activity of 17-O-Acetylacuminolide through the Inhibition of Cytokines, NF-κB Translocation and IKKβ Activity
Figure 7
Effect of AA on iNOS expression in RAW264.7 cells.
In untreated cells, the nuclei of the cells appear green (hoescht) and iNOS expression was undetected (DMEM). iNOS (DyLight™ 488, magenta), was expressed in the cell cytoplasm of stimulated cells. Pretreatment with aminoguanidine (AG) or 17-O-acetylacuminolide (AA) was able to inhibit iNOS expression in cells stimulated with lipopolysaccharide and interferon gamma (LPS+IFNγ).