In Vitro and In Vivo Anti-Inflammatory Activity of 17-O-Acetylacuminolide through the Inhibition of Cytokines, NF-κB Translocation and IKKβ Activity
Figure 6
Percentage inhibition of nitric oxide (NO) in RAW264.7.
Cells were pretreated with the indicated doses of 17-O-acetylacuminolide (AA) or with 1 mM of the iNOS inhibitor Aminoguanidine (AG). The cells were stimulated with LPS and IFNγ to activate iNOS synthesis, and eventually NO release. Data presented is the percentage of NO inhibition compared to untreated (DMEM), stimulated cells (control). Data is the average of three independent experiments (±SD), and was analyzed using one way ANOVA with Tukey's post test (* p<0.05, ** p<0.01, *** p<0.001).