C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
Figure 2
Spatial distribution of GFP-Tud fusion proteins during oogenesis.
GFP-Tud proteins were detected in fixed egg chambers. Panels of the left display previtellogenic egg chambers whereas panels on the right exhibit vitellogenic egg chambers. (A) The GFP-Tud-JOZ fusion protein accumulated in nurse cell and oocyte nuclei. In a stage 10 egg chamber no localization of this protein could be detected at the posterior pole of the oocyte. (B) The GFP-Tud-9A1 fusion protein could be found in the nuage and dispersed in punctuate structures in the cytoplasm. In stage 10 egg chamber the fusion protein accumulated at the posterior pole of the oocyte. (C) The GFP-Tud-3ZS+L fusion protein was present in both nuage and oocyte of previtellogenic egg chambers and localized in the pole plasm of a late stage 9 egg chamber. (D–E). Both GFP-Tud- 3ZS+L-N and GFP-Tud-3ZS+L-C fusion proteins corresponding to the N- and C-moieties of GFP-Tud-3ZS+L, respectively, displayed a pattern of distribution in both nuage and pole plasm similar to the original GFP-Tud- 3ZS+L fusion protein with the exception that the staining intensity was lower and that the pole plasm accumulation of the N-terminal moiety was significantly reduced.