C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
Figure 1
Synthesis of GFP-Tud fusion proteins in Drosophila ovaries.
(A) Representation of the Tud protein with Tudor (1–8) and Tudor-like (1′-2′) domains depicted in purple. Fragments of Tud [33] used to design the transgenes are indicated below the map. (B) (Upper panel) Western blot analysis of GFP-Tud fusion proteins synthesized in ovaries of transgenic females using anti-GFP antibodies followed by alkaline phosphatase-conjugated antibodies. (Lower panel) The blot was then probed for ribosomal P40, as loading control.