Interaction with Diurnal and Circadian Regulation Results in Dynamic Metabolic and Transcriptional Changes during Cold Acclimation in Arabidopsis
Figure 3
Effect of cold on cyclic gene expression and metabolite content.
(A) Venn diagram showing the number and overlaps between of transcripts classified into each of the following three groups: 1) diurnally regulated at 20°C, 2) circadian regulated at 20°C or 3) cold-responsive. Circadian and diurnal regulated transcripts were identified using the HAYSTACK tool or by autocorrelation, respectively. Cold responsive genes were identified using limma with a sliding window of three sequential time points (FDR<0.05) (see Methods for details). (B) Venn diagram showing the number and overlaps between metabolites classified into each of the following four groups: 1) diurnally regulated at 20°C, 2) circadian regulated at 20°C, 3) diurnally regulated at 4°C, 4) circadian regulated at 4°C. Circadian and diurnal regulated transcripts were identified using the HAYSTACK tool or by autocorrelation, respectively (see Methods for details).