Suppression of Hypoxia-Inducible Factor 1α (HIF-1α) by Tirapazamine Is Dependent on eIF2α Phosphorylation Rather Than the mTORC1/4E-BP1 Pathway
Figure 6
The effect of TPZ on HepG2 human xenograft models.
The mice transplanted with HepG2 human xenograft were randomly divided into two groups and given injection of TPZ (30 mg/kg) or vehicle every 2 days. Representative photomicrographs (magnification, x100) showing immunofluorescence staining for HIF-1α (A) and p-eIF2α (B) in tumor sections of vehicle–treated and TPZ-treated mice. The immunostaining data were confirmed in two or more specimens of each group. (C) Tumor volume are expressed as mean ± SD. (D) Relative tumor volume are expressed as mean ± SD. (**p<0.01, relative to vehicle group).