Loss of LMO4 in the Retina Leads to Reduction of GABAergic Amacrine Cells and Functional Deficits
Figure 7
Loss of Bhlhb5+ cells in P0 LMO4 cko retinas.
(A) MO4 is colocalized with Bhlhb5 in amacrine cells (arrows). LMO4 mRNA in the cytoplasm was revealed by in situ hybridization with an antisense probe labeled with biotin (green). Bhlhb5 in the nuclei was revealed by an antibody to Bhlhb5 (red). Inset is enlarged in the lower panels. Scale bars = 100 and 25 µm, top and bottom panels, respectively. (B) Bhlhb5 immunostained cells were significantly fewer in the peripheral retina of LMO4 cko mice compared to littermate controls. Inset is enlarged in the lower panels. Scale bar = 100 µm.