Loss of LMO4 in the Retina Leads to Reduction of GABAergic Amacrine Cells and Functional Deficits
Figure 6
Quantitation of Bhlhb5+ amacrine and bipolar cells in P15 retinas.
Immunopositive cells were counted at the peripheral retinas, within 500 µm from the tip, in the plane of the optic nerve. (A) A reduction in the density of amacrine cells labeled with the pan-amacrine Pax6 antibody and Bhlhb5 antibody that labels GABAergic amacrine cells was measured in LMO4 cko mice compared to littermate controls. No difference in cholinergic (ChAT+) or dopaminergic (TH+) amacrine cell density was observed. (B) A reduction in the density of bipolar cells labeled with the pan-bipolar Chx10 antibody and Bhlhb5+ cone bipolar (CB) cells was observed in LMO4 cko mice. However, PKC+ rod bipolar (RB) cell density was not different. Filled bars are for littermate controls and open bars are for LMO4 cko mice. The bars indicate s.e.m. and the asterisks indicate p<0.05.