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The Armadillo Repeat Protein PF16 Is Essential for Flagellar Structure and Function in Plasmodium Male Gametes

Figure 2

Plasmodium PF16 is expressed in the male gametocyte and gametes.

Immunolocalisation of GFP in PF16-GFP-expressing parasites, both in the male gametocyte (0 min) and during male gamete formation during the exflagellation process (5–15 min). The parasites were co-stained with an anti tubulin antibody (red). Hoechst staining (blue) indicates the presence of DNA. Note the increase in the intensity of GFP localisation with the increase in time, and at the end of exflagellation when motile gametes form (15 min). Scale bar, 5 µm.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0012901.g002