Antibody Targeting of Cathepsin S Inhibits Angiogenesis and Synergistically Enhances Anti-VEGF
Figure 4
Fsn0503 in combination with an anti-VEGF antibody synergistically inhibit angiogenesis in vitro.
(A) Representatives tube assay images of VEGF control, anti-VEGF antibody treated, Fsn0503 treated and the combination of anti-VEGF antibody and Fsn0503 which shows the greatest disruption of tube formation. (B, C) Tube formation was assessed by counting nodes with 1, 2, 3, or more branches and the average calculated per field of view. The number of nodes with 3 or more branches, which are indicative of normal tube formation were assessed to demonstrate efficacy. Quantification of tube formation shows the single agents reducing the number of nodes with 3 or more branches and the combination synergistically reducing the number of nodes with 3 or more branches and increasing the number of single branches.