Calcineurin Interacts with PERK and Dephosphorylates Calnexin to Relieve ER Stress in Mammals and Frogs
Figure 5
Knockdown of CN-A attenuate the protein synthesis inhibition under ER stress.
(A) CN-A expression levels are shown for the following conditions: control oocytes (lanes C and C-tg)(Cntrl, lane 1), oocytes injected with standard control morpholinos (lanes S and S-tg) (Std Morpho, lane 2), oocytes injected CN-A morpholinos (lanes M and M-tg) (Morpho CN 1& 2, lane 3). A subgroup of each oocyte pool was also stressed for 30 minutes with Tg (lanes C-tg, S-tg and M-tg 4–6). Note that CN is decreased by the CN-A morpholinos treatment only after ER stress (lane M-tg 6). Expression levels are indicated by Western blot (top panels). Loading controls are presented in the bottom panels. All bands were from the same gel and received the same exposure time. (B) Histogram depicts the mean intensity of each band relative to the corresponding density in the Western blots of CN. Data pooled from three independent experiments (n = 3). (C) Autoradiography of total protein synthesized in control oocytes or injected with morpholinos as was described above, that has been untreated or exposed to Tm before a 45 minutes pulse label with [35S]-Methionine-Cysteine. The two panels are from the same gel and received the same exposure time. (D) Histogram corresponding to densitometric analysis of total protein. Data pooled from three independent experiments (n = 3).