Epstein-Barr Virus Interferes with the Amplification of IFNα Secretion by Activating Suppressor of Cytokine Signaling 3 in Primary Human Monocytes
Figure 5
siRNA against SOCS3 restores IFNα secretion following EBV infection.
Monocytes (2×106 cells) were transfected with 165 nM siRNA targeting SOCS1 or SOCS3. Scramble siRNA was used as control. Twenty-four hours after transfection, cells were stimulated with EBV for 20 hours (1st EBV stimulation). Following this first EBV stimulation, medium was replaced and cells were stimulated a second time with EBV (2nd EBV stimulation) for an additional 20 hours. Cell-free supernatants were then harvested for IFNα determination. Data are representative of three independent experiments. Values in percentage represent the inhibition in IFNα secretion relative to the first respective EBV stimulation. *p≤0.05 compared to cells transfected with scramble siRNA and stimulated a second time with EBV. SCR: scrambled siRNA.