Epstein-Barr Virus Interferes with the Amplification of IFNα Secretion by Activating Suppressor of Cytokine Signaling 3 in Primary Human Monocytes
Figure 4
Effect of EBV infection on IRF3 and IRF7 activation.
1st EBV stimulation: Monocytes (5×106 cells) were stimulated or not with EBV for the indicated times and expression of phosphorylated forms of IRF3 and IRF7 proteins was evaluated by Western blot analysis. 2nd EBV stimulation: Monocytes were first stimulated with EBV for 20 hours, washed and then restimulated a second time with EBV for the indicated times. Expression of phosphorylated forms of IRF3 and IRF7 proteins was evaluated by Western blot analysis. Membranes were also probed with anti-Actin as a loading control. Densitometry was performed and represents fold protein induction (relative to 0 h) ± std. dev. of experiments performed in duplicate. Data are representative of three independent experiments.