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DCB-3503, a Tylophorine Analog, Inhibits Protein Synthesis through a Novel Mechanism

Figure 4

DCB-3503 preferentially inhibited [14C]-thymidine incorporation in a time- and dose-dependent manner, but not [14C]-uridine incorporation.

A. and B. The inhibitory effect of DCB-3503 on [14C]-thymidine incorporation was time-dependent and dose-dependent in HepG2 cells. (C) and (D) DCB-3503 showed less than 50% inhibition on [14C]-uridine incorporation in either HepG2 cells followed by different time and dose treatment. Hydroxyurea (Hu) and actinomycin D were used as positive controls. Values are depicted as mean ± S.D. from at least three separate experiments.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0011607.g004