Double Negative (CD3+4−8−) TCRαβ Splenic Cells from Young NOD Mice Provide Long-Lasting Protection against Type 1 Diabetes
Figure 1
Frequency of NOD DNCD3 thymic and splenic cells.
Total thymocytes and splenocytes from individual NOD and NON.NOD control males and females were harvested at various time-points after birth, stained with CD3-PE, CD4-PE-Cy7, and CD8-PerCP mAb-conjugates, and the frequency of CD3+4−8− (DNCD3) cells was measured by FACS. Panel A, cells gated based on the forward and light scatter to identify viable populations. Panel B, kinetics of thymic vs. splenic DNCD3 cell development in newborn NOD mice. Panel C, kinetics of thymic vs. splenic DNCD3 cell development in newborn NON.NOD control mice. Shown are the individual variations (mean ± SD) in each age group.