Modeling Susceptibility versus Resistance in Allergic Airway Disease Reveals Regulation by Tec Kinase Itk
Figure 5
Itk regulates the development of susceptibility or resistance to allergic airway inflammation dependent on background.
A) Model of data analysis. Strain 1 and 2 differ with regards to their dose response to allergen. This difference can be captured by the slope of their dose response (Left panel), the significance of which can inform on whether there is a response (p<0.05) or not (p>0.05) (right panel). These p values can be used as described in the text to determine resistance (R), i.e. no response (with p>0.05,) or the degrees of susceptibility (with p<0.05), which is related to the slope of their response, and thus the p values of those slopes. B) Analysis of the slopes of the IL-4, IL-13 and IFN-γ (left panel) or CCL-7 and CCL-11 (middle panel) dose responses for the indicated mice. Analysis of the inverse of the slopes of the data shown in (A) (right panel, note log scales on the y-axis). C) Analysis of Susceptible (Saai) or Resistance (Raai) as described in thematerials and methods . The dotted line indicates the cutoff of 0.77 between resistance and susceptibility. D) Hierarchical clustering of the values in (C) to generate a cluster tree (B6 = C57BL/6; Bc = BALB/c; B6/Itk−/− = C57BL/6/Itk−/−; Bc/Itk−/− = BALB/c/Itk−/−).