An Antimicrobial Peptide Regulates Tumor-Associated Macrophage Trafficking via the Chemokine Receptor CCR2, a Model for Tumorigenesis
Figure 6
HBD-3 induced cytokines macrophages.
(A and B) Macrophages differentiated from THP-1 cells using PMA stimulation (A) and from peripheral blood monocytes (PBMs) using macrophage-colony stimulating factor (M-CSF) treatment (B) [65]. Note macrophage-like morphological changes after differentiation. (C) Real-time quantitative RT-PCR of IL-1α, IL-6, IL-8, CCL18, and TNF-α in THP-1 cell-differentiated macrophages treated with hBD-3 (10 µg/ml) for 16 h. (D) Real-time quantitative RT-PCR of IL-1α, IL-6, IL-8, CCL18, and TNF-α in PBM-differentiated macrophages treated with hBD-3 (10 µg/ml) for 16 h. Experiments were repeated 3 times. p values are presented in each graph.