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A Distinct Expression Pattern in Mammalian Testes Indicates a Conserved Role for NANOG in Spermatogenesis

Figure 4

Expression of NANOG in dog testis as determined by immunofluorescence.

(A–F) NANOG expression in differentiating male germ cells. Roman figures in panels represent the stage of the canine epithelial cycle (G–I) high magnification image of section in which the primary antibody was replaced by blocking solution, to control for non-specific binding of the secondary antibody; (J–L) image of testis section that was incubated with a rabbit IgG isotype control, to control for non-specific binding of the primary antibody.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0010987.g004