A Distinct Expression Pattern in Mammalian Testes Indicates a Conserved Role for NANOG in Spermatogenesis
Figure 2
Expression of NANOG and eGFP in mouse testes sections as detected by immunohistochemistry.
(A–C) Expression of NANOG in mouse testis at different stages of spermatogenesis; Roman figures in seminiferous tubules represent the stage of the epithelial cycle; (D) Negative control section from which the primary antibody was omitted in the staining procedure; (E) Expression of eGFP in testis sections of a Nanog eGFP reporter; (F) eGFP staining on testis sections of a non-transgenic mouse, which served as a negative control for the staining in figures E. Abbreviations: AS = type A spermatogonia, P = Pachytene spermatocyte, T = Spermatid, ET = elongating spermatids, S = Sertoli cell, * = non-specific binding to interstitial cells. Sections displayed in panels A–D were fixed in Bouins fixative; sections in panels E and F were fixed in formalin. Formalin fixation interfered with immunostaining of the seminiferous tubules.