Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity
Figure 5
Time-dependent reporter gene activation in an ex vivo human skeletal muscle cell differentiation system.
(A) Phase contrast microscopy of co-cultures containing myoblastsFLPe and myoblastsGS.Luc at a 1∶1 ratio after incubation for 2, 3, 4 or 5 days in growth medium (upper panels) or in differentiation medium (lower panels). (B) Luminometric analysis of cell lysates prepared at 12-hour intervals from co-cultures initiated with 5×104 myoblastsFLPe and with 5×104 myoblastsGS.Luc (upper panels) or with 105 myoblastsFLPe and with 105 myoblastsGS.Luc (lower panels). The cells were either maintained in growth medium (solid bars) or in differentiation medium (open bars). The relative light units (RLU) are plotted on linear (left panels) and logarithmic (right panels) scales. Bars correspond to mean ± standard error of the mean (n = 3).