HDAC6 Regulates Mitochondrial Transport in Hippocampal Neurons
Figure 6
5-HT signals increase levels of Kinesin-1B and acetylated tubulin in mitochondrial fractions isolated from hippocampal neurons.
A, C. 5-HT or 8-OH-DPAT increases acetylation of tubulin in hippocampal neurons. B, D. Fluoxetine increases acetylation of tubulin in hippocampal neurons. E, F, G, H. 5-HT, 8-OH-DPAT, or fluoxetine increase the amount of kinesin1-B (top panel) and acetylated tubulin (bottom panel) found in a mitochondrially-enriched fraction. Mitochondrial protein levels were normalized to VDAC. Quantifications of Western blots were based on multiple experiments (n≥3).