A Three-Dimensional Atlas of the Honeybee Neck
Figure 10
The steps involved in generation of the three-dimensional atlas.
(A) Imaging: multiple images of each section were stitched together to create a single high-resolution image. Outline of multiple images are visible as white boundaries on the black background. (B) Alignment: images of each section were aligned relative to each other. Registration was quickly verified by volume rendering the image stack; the bee head and thorax are shown from the top (left) and side (right). (C) Segmentation: every structure of interest was manually outlined in each of the aligned sections. Different colours overlaid over this cross-section of the prothorax represent different structures. The boxed area illustrates the region shown in Fig. 11. (D) Model generation: mesh models were created from stacks of segmented images using Amira 3.1. (E) Redrawing: mesh models were greatly smoothed, simplified, and corrected for artefacts by manually redrawing using Silo 2.1. The approximate total number of polygons in each of the mesh models is indicated by n.