Agrin Binds BMP2, BMP4 and TGFβ1
Figure 5
Effect of Agrin-Nterm on the binding of BMP2, BMP4 and TGFβ1 to the extracellular domain of their cognate receptors, monitored by surface plasmon resonance.
A) Sensorgrams of the interactions of immobilized ECD of BMPRIA with 40 nm BMP2 preincubated with 0 nM, 12 nM, 29 nM, 58 nM, 88 nM, 146 nM and 293 nM of Agrin-Nterm. B) Sensorgrams of the interactions of immobilized ECD of BMPRIA with 40 nm BMP4 preincubated with 0 nM, 220 nM, 440 nM, 660 nM, 880 nM, 1100 nM and 1540 nM of Agrin-Nterm. C) Sensorgrams of the interactions of immobilized ECD of TGF-βsRII with 4 nm TGFβ1 preincubated with 0 nM, 912 nM, 1824 nM, 2736 nM and 4560 nM of Agrin-Nterm. Growth factors were preincubated with Agrin-Nterm in 20 mM HEPES buffer, pH 7.5 containing 150 mM NaCl, 5 mM EDTA, 0.005% Tween20 for 30 min at room temperature and were injected over CM5 sensorchips containing immobilized ECD of receptors. For the sake of clarity the concentrations of Agrin-Nterm are not indicated in the panels; in each case SPR response decreased parallel with the increase of Agrin-Nterm concentration. Panels on the right indicate the concentration dependence of the inhibitory effect of Agrin-Nterm as monitored by changes in observed association rate, kobs.