Cannabinoid-Mediated Inhibition of Recurrent Excitatory Circuitry in the Dentate Gyrus in a Mouse Model of Temporal Lobe Epilepsy
Figure 5
Effect of cannabinoid agonists on TTX-independent mEPSCs in pilocarpine-treated mice that survived SE.
A. mEPSCs recorded from a granule cell in the presence of TTX (1 µM). B. Trace showing the reduction of mEPSC frequency after application of AEA (10 µM). C. Wash to TTX alone (20 min) showing that the effect was reversible. A1, A2, B1, B2 and C1, C2 are expanded segments of A, B and C, respectively. Recordings were made in the presence of bicuculline (30 µM). D. Cumulative probability plot showing a change in the frequency of mEPSCs in this cell. E and F. Cumulative charts showing effect of AEA, WIN 55,212-2 and 2-AG on mEPSC frequency (E) and amplitude (F) on pilocarpine-treated (TLE) and control mice. Asterisk indicates significant change in mEPSC frequency (p<0.05); number of recordings shown in parentheses.