Cannabinoid-Mediated Inhibition of Recurrent Excitatory Circuitry in the Dentate Gyrus in a Mouse Model of Temporal Lobe Epilepsy
Figure 2
Increase in synaptic activity in granule cells from pilocarpine-treated mice that survived SE and developed TLE.
A. sEPSCs recorded from granule cell of normal mouse. B. sEPSCs recorded from granule cell of a mouse that was injected with pilocarpine but did not develop SE. C. sEPSCs and bursting activity recorded from a granule cell of a pilocarpine-treated mouse that developed TLE. D. Trace showing that sEPSCs were blocked by application of ionotropic glutamate receptor antagonists, CNQX (10 µM) and AP-5 (50 µM). A1 and B1 are expanded boxed regions of A and B. C1, expansion of the sEPSC showing increased synaptic activity during the burst seen in C. Recordings were made in the absence of added Mg2+ and the presence of bicuculline (30 µM). E. Bar graph comparing the frequency of sEPSCs among the three groups of animals. Asterisk indicates significantly higher frequency in cells from mice with TLE (P<0.05).