A Locked Nucleic Acid Antisense Oligonucleotide (LNA) Silences PCSK9 and Enhances LDLR Expression In Vitro and In Vivo
Figure 2
Effect of LNA ASO on mRNA expression of LDLR.
Human hepatic HepG2 and HuH7 and mouse insulinoma β-TC3 cells were transfected with LNA ASO at concentrations of 10 nM and 25 nM, and the levels of LDLR mRNA analyzed by QPCR after 24 h and 48 h. The levels of LDLR mRNA were normalized to (A and B) S14 mRNA and (C) S16 mRNA for human and mouse origin, respectively.