Synthesis of 5-Hydroxyectoine from Ectoine: Crystal Structure of the Non-Heme Iron(II) and 2-Oxoglutarate-Dependent Dioxygenase EctD
Figure 6
The ectoine hydroxylase signature sequence motif represented in the context of the EctD crystal structure.
The V. salexigens EctD structure is shown in ribbon representation with those residues constituting the EctD signature motif [F143-X-WHSDFETWH-X-EDG-M/L-P159] highlighted in yellow. This string of amino acids is invariantly present in 71 compiled EctD-type proteins (Figure S1). The side chains of the Fe2+-chelating amino acids (His-146, Asp-148, His-248) are shown as red sticks and those forming the putative 2-oxoglutarate-binding site (Phe-143, Ser-250, Arg-259) are represented by green sticks. The iron ligand (Fe3+) present in the EctD crystal structure is represented as blue sphere.