R-Flurbiprofen Reduces Neuropathic Pain in Rodents by Restoring Endogenous Cannabinoids
Figure 6
Immunofluorescence analysis of microglia in the spinal cord dorsal horn seven days after nerve injury.
Microglia was identified by Iba-1 immunoreactivity (green). Mice were treated with R-flurbiprofen (4.5 mg/kg twice daily, left panel) or vehicle (right panel). Figures show the side ipsi lateral to the nerve lesion (a, c, e, f, g, h, i, j, k, l) and the contra lateral side (b, d). The inserts in i and j are z-projections. In I, J neurons were visualized by NeuN immunofluorescence (red). In k, l astrocytes were stained for glial fibrillary acidic protein (GFAP, red). Scale bars: 100 µm in a–f; 50 µm in g–l; 20 µm in the inserts of I, j.