R-Flurbiprofen Reduces Neuropathic Pain in Rodents by Restoring Endogenous Cannabinoids
Figure 1
Time course of the nociceptive behavior in the Spared Nerve Injury (SNI, left panel) and the Chronic Constriction Injury (CCI, right panel) model of neuropathic pain.
Rats were treated with R-flurbiprofen, S-flurbiprofen, gabapentin or vehicle (n = 6 per group). The daily treatment (twice daily i.p.) started 10 days after nerve injury. a Mechanical hyperalgesia was assessed by recording the paw withdrawal threshold to stimulation with a Dynamic Plantar Aesthesiometer, b mechanical allodynia as paw withdrawal threshold to von Frey hairs, c, d cold allodynia as response time in the acetone test and e, f cold hyperalgesia by counting withdrawal reactions during exposure to a cold plate at 2°C. Comparison of the areas under the effect x time curves revealed statistically significant differences between R-flurbiprofen and gabapentin treated animals compared with vehicle or S-flurbiprofen. For R-flurbiprofen 4.5 or 9 mg/kg twice daily provided statistically significant antinociceptive effects. For SNI, respective P values were 0.002 for mechanical, 0.026 for cold allodynia, 0.018 for cold hyperalgesia. For CCI, P values were 0.001 mechanical, 0.041 cold allodynia, n.s. for cold hyperalgesia.