The Yeast Cell Fusion Protein Prm1p Requires Covalent Dimerization to Promote Membrane Fusion
Figure 4
Prm1p localization and self-association are not affected by cysteine substitution.
(A) Transmitted light and epifluorescence images of live mating pairs shortly after coupling. Prm1p and indicated alleles were C-terminally tagged with GFP. (B) MATa cells expressing epitope tagged PRM1 or mutant alleles as indicated were mated to wild-type MATα cells on YPD plates at 30°C for 3 h. Myc-tagged fusion proteins were immunoprecipitated using α-myc-Agarose.