The Yeast Cell Fusion Protein Prm1p Requires Covalent Dimerization to Promote Membrane Fusion
Figure 2
Prm1p forms covalent homodimers.
MATa and MATα cells expressing epitope tagged PRM1 as indicated were mated on YPD plates at 30°C for 3 h. After cell disruption, membrane proteins were solubilized in 1% Triton X-100 and immunoprecipitated using α-myc-Agarose. Western blotting with α-myc and α-GFP antibodies was used to assay pull-down efficiency and co-immunoprecipitation.