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Rac1 Is Required for Epithelial Stem Cell Function during Dermal and Oral Mucosal Wound Healing but Not for Tissue Homeostasis in Mice

Figure 2

Deficient epithelial tongue migration in K14CreRac1F/F mice.

(A) Control mice. Immunofluorescence for cytokeratins 10 and 14 (K10/14, red) reveals the epidermal layer of the skin and the epithelial tongue migrating underneath the fibrin clot at day 6 (Green). DNA staining (Hoechst 33342-blue) delineates cellular nuclei. Note the extensive migration of the epithelial tongue in merged figure (high Magnification-20x). (B) K14CreRac1F/F conditional knockout mice stained for cytokeratins 10 and 14 (K10/14, red) show a more limited migration of the epithelial tongue by day 6 after injury. The deficient migration of the epithelial tongue is visualized in the merged figure (High magnification-20x).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0010503.g002